lSodium dodecyl sulphate-polyacrylamide gel electrophoresis or SDS-PAGE is commonly used in protein aboratories for separating proteins based on their molecular weights. This is accomplished by taking advantage of their differential rates of migration through a sieving matrix (the acrylamide or agarose gel) in response to an applied electrical field.
0 Comments Click here to read/write comments
Topics: Protein Electrophoresis
Most biological samples contain non-protein substances and/or contaminants that may interfere with the resolution of the electrophoretic separation. Considering the fact that cell lysates usually contain macromolecules and small ionic molecules which may interfere with the electrophoretic process, these agents should be eliminated prior to electrophoresis, especially if their amount exceeds the critical interference threshold.
0 Comments Click here to read/write comments
Topics: Protein Electrophoresis
Question:
How to Determine Molecular Weight of Protein with SDS-Page?
12 Comments Click here to read/write comments
Question:
How 2DE Protein Electrophoresis works?
0 Comments Click here to read/write comments
Topics: Protein Electrophoresis
