The Protein Man's Blog | A Discussion of Protein Research

Multiplex PCR is a technique used to amplify multiple target sequences in a single PCR reaction using multiple primer sets. Multiplex PCR was first reported by Chamberlain et al. in 1988. This method is used to detect deletions, polymorphisms, mutations, etc., This method is also used to detect different viral, bacterial and other pathogens in a single tube. This method consumes less time and effort in obtaining the results, but problems posed during multiplex PCR include poor sensitivity, specificity, and problems in amplification of some specific targets.

While phenol extraction is one of the commonly used methods in purifying DNA samples (i.e., removing proteins from cell lysates during DNA preparation), some people do not fully understand how and why it works. Read on to learn and appreciate how phenol extraction works.

Loop mediated isothermal Amplification (LAMP) technique is one of the recent PCR techniques that is gaining importance for its application in molecular diagnostics. LAMP is a single tube PCR reaction carried out at constant temperature. LAMP is simple, affordable, cost effective, requires simple machinery and can be used in remote places by persons with little training.

There has been an exponential interest developing from past few years for studying the translational regulation of different genes in many model systems. Protein synthesis is the functional and fundamental biological process essential for decoding the genetic information and culminates it with a specific phenotype. Since the energy expenditure on protein synthesis is roughly more than half of the total energy expense of the cell during rapid growth, thus studying the kinetics of translational instrumentation has gained a broad attraction recently. As it has been shown previously, even a subtle altercation in translational apparatus can substantially affect human health. Hence, it is essential to profile the actively translating proteins comprehensively in order to develop insights into the cumulative translatome and its functional correlations.