Enzyme-Linked Immunosorbent Assay (ELISA) is one of the most widely used techniques in immunology, molecular biology, and clinical diagnostics. This powerful method allows researchers to detect and quantify proteins, antibodies, hormones, and other biomolecules with high specificity and sensitivity. Antibodies and enzyme-mediated reactions generate a signal, reflecting the presence and amount of the target substance. However, achieving accurate and reproducible ELISA results requires careful optimization and attention to detail. In this blog, we explore key considerations for optimizing ELISA experiments, highlight common pitfalls to avoid, and recommend ELISA kits, buffers, and detection reagents to ensure success.
