Viability assays are strategies and techniques that are generally used to determine whether or not an organ, cell, or tissue remains viable or could potentially recover its viability. In general, viability assays will look at the cells or tissue involved to see if they still react and move as they are supposed to. Mitotic activity, mechanical activity, and motility are all used throughout a viability assay for this reason.
When proteins need to be segregated out from a sample, protein electrophoresis may be used. Protein electrophoresis utilizes a matrix and an electrical current in order to easily separate proteins without disruption. Protein electrophoresis is beneficial for a few reasons: it can be performed on a relatively small sample size, it is accurate, and it is simple. There are a few different methods of protein electrophoresis, which may or may not involve a denaturing gel.
Glutathione assay involves quantification of reduced Glutathione (GSH) and Oxidized Glutathione (GSSG) in various organisms or in various tissues, blood samples, plasma, serum or cultured cells. Free thiols such as GSH can be detected by their property of relatively high reactivity compared to other biological molecules. On contrary disulfides such as GSSG does not have any unique property that could be exploited for its quantification. Hence Glutathione disulfide (GSSG) quantification can be done after reducing it to its corresponding thiol (GSH). Thus the most widely adopted methodology for quantification of GSH and GSSG is determination of total GSH concentration, followed by alkylation to remove GSH and then reduction of GSSG and its quantification. GSH concentration in sample can be determined by subtracting glutathione disulfide concentration from total glutathione concentration.
Topics: Apoptosis Assays
Monitor oxidative stress to determine the extent of cell damage using a colorimetric gluthathione assay.
Topics: Cytotoxicity Assays
