The Protein Man's Blog | A Discussion of Protein Research

Ammonium Sulfate Protein Precipitation: The key to Salting-Out

Posted by The Protein Man on Sep 6, 2017 2:30:00 PM
The Protein Man

Salting-Out.pngSalt precipitation can be a very powerful tool to purify proteins by precipitation. Ammonium sulfate is usually the salt of choice since it is cheap, very soluble in water, and is able to become much more hydrated (interacts with more water molecules) than almost any other ionic solvent. In practice, ammonium sulfate is either added directly as a solid or added as a (usually) saturated solution to precipitate desired proteins.

At low salt concentrations (<0.15M) the addition of more salt in general tends to increase the solubility of proteins as ions shield the protein molecules from the charges of other molecules; this trend is termed ‘salting-in’. At some point the ionic strength becomes too high and starts to have a negative effect on the solubility of proteins, termed ‘salting-out’. This happens because the dissolved salt competes with the proteins for scarce water molecules, increasing the surface tension of water and therefore causing the protein to fold tighter. The reduction in protein surface area means less protein-water interactions which allows for more hydrophobic interactions between protein molecules, causing aggregation and subsequently precipitation.

Proteins in solution can also be fractionated out since they will precipitate out as a function of salt concentration. In this way it is possible to purify specific proteins by adding a specific amount of ammonium sulfate to precipitate out non-desirable proteins, recovering the supernatant, and then adding a bit more ammonium sulfate to precipitate out the desired protein and then save that pellet of precipitated protein. Since salt precipitation only affects the solubility of proteins and does not denature them, the recovered fraction can be stored in the salt solution for prolonged periods of time without having to worry about bacterial contamination since the high salt content inhibits any microbial growth or protease activity.

Because ammonium sulfate precipitation only reduces the solubility of proteins and does not denature them proteins can be concentrated by removing the remaining ammonium sulfate solution then the protein pellet can be resolubilized in standard buffers or a lower concentration of ammonium sulfate. Hydrophobic interaction chromatography or gel filtration chromatography can then be used to further purify the protein solution. Ammonium sulfate can also be used to guide some proteins unfolded by denaturants such as urea back to their proper native conformations by gradually increasing the concentration of ammonium sulfate.

There are some best practices that should be considered when working with ammonium sulfate such as:

  • Use a mortar and pestle to break up clumps and otherwise grind up the ammonium sulfate to facilitate addition and dissolution.
  • Add only a small amount of ammonium sulfate at a time, wait for it to dissolve and gently stir to avoid foaming.
  • Use a buffer such as 50 mM HEPES or Tris to minimize the acidifying nature of ammonium sulfate.
  • Use analytical grade ammonium sulfate since lower grades typically exhibit heavy metal contamination.

The process of salt precipitation does come with some disadvantages. Salting out proteins requires prior knowledge of the protein’s solubility. In addition, any contaminants present in the initial sample may still be present in the fraction containing the protein of interest; this process concentrates the protein but does not purify it. Furthermore, it may be necessary to remove the salt from the protein sample and so further processing in the form of either dialysis or chromatography will be required.

Related Blog Posts

Topics: Protein Purification, Protein Concentration

Want more Protein Man blogs?

Ellyn Daugherty's Biotechnology: Science for the New Millennium

CB™ PROTEIN ASSAY: A Bradford Protein Assay

CB Protein Assay Graph

An improved Coomassie Dye based protein assay based on the Bradford Protein Assay. This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color from brown to blue. The change in color density is proportional to protein concentration. Protein estimation can be performed using as little as 0.5µg protein.


  • Sensitivity: Linear responses over the range of 0.5µg-50µg protein
  • Flexible Protocols: Suitable for tube or Titer plate assays
  • Ready to use assay reagents and no preparation required
  • Long shelf life, stable for 12 months
Read More