The Protein Man's Blog | A Discussion of Protein Research

Fast Flow Agarose Resins: What's The Real Difference?

Posted by The Protein Man on Jul 14, 2016 10:00:00 AM
The Protein Man

gbio123.jpgFor the purposes of chromatography, fast flow agarose resin provides a stable, easy, and effective medium. Comprised of cross-linked agarose beads, fast flow agarose resin is able to improve upon both the speed and reproducibility of chromatography, offering good resolution and the ability to predictably scale up. Fast flow resins also have extremely good flow properties and superior loading capacity, making it ideal for faster separation cycles, better purification, and lessened dilution.

Higher Chemical and Physical Stability

In gel filtration, resin beds are required to remain stable and avoid reactions. Fast flow agarose resin have a solid chemical stability, which provides for better sanitization and recovery throughout numerous purification cycles, including Cleaning In Place (CIP). A 0.5 to 1.0 sodium hydroxide wash can be used to clean fast flow agarose resin in most situations. This chemical stability also offers better reliability and speed, making them the resin of choice for laboratories that need to be able to reproduce their results with a high degree of accuracy. Fast flow agarose resins can be scaled up very quickly through the use of pre-packed chambers. Additionally, many forms of fast flow resin have a hydrophilic nature, which inclines them to lower potential levels of impurities from the host solution.

Easy Scale Up With Fast Flow Resin

The ability of fast flow resin to easily scale up makes it particularly useful for preparatory applications and laboratory-scale processes. Fast flow resin can be used for process development, routine operations, and scaled up processes, all while still maintaining reliability and reproducibility. Multiple forms of fast flow resin are available for different applications and needs, often depending on the size of the sample that is required and the sample applications. Even better, fast flow resin can reliably scale up without increasing the dilution or inaccuracy of the given sample.

Pre-Packed Fast Flow Resin Chambers

Fast flow resin is available pre-packed in chambers for individuals who want to be able to work quickly and with minimal preparation. These resin chambers are designed for a broad spectrum of requirements and are incredibly easy to process. Laboratories that prefer to pack their own columns can instead purchase the resin medium directly. Fast flow agarose resins generally come with complete recommendations regarding how they should be packed, ensuring the best and most consistent results. The available pre-packed chambers are specially designed to meet most general purpose separation and exchange needs.

Industry Standard for Commercial and Industrial Use

Fast flow agarose resins have become a popular industry standard for commercial, industrial, and laboratory use. These resins are presently preferred for the use of purification, filtration, and capture, and are able to provide preparatory protein separations for a multitude of applications. As an industry standard, fast flow agarose resin is well-supported and offers a myriad of different types that are designed for numerous specific applications. For the purpose of protein separation and exchange, fast flow agarose resins are one of the fastest, most stable options.

Fast flow agarose resin is a versatile, all-purpose solution that can be used any time a filtration medium is required. It is primarily useful for separating protein mixtures and general preparative protein separations. In any case when gel filtration may be required, fast flow agarose resin can be used as a reliable, stable, and scalable resin bed. When necessary, fast flow agarose resin can be scaled up five-fold or twenty-fold using only pre-packed columns. Finally, fast flow agarose improves upon both the speed and reliability of these results, making it the ideal resin bed for the purposes of filtration.

Want more Protein Man blogs?

Purification_resins
Ellyn Daugherty's Biotechnology: Science for the New Millennium

CB™ PROTEIN ASSAY: A Bradford Protein Assay

CB Protein Assay Graph

An improved Coomassie Dye based protein assay based on the Bradford Protein Assay. This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color from brown to blue. The change in color density is proportional to protein concentration. Protein estimation can be performed using as little as 0.5µg protein.

Features

  • Sensitivity: Linear responses over the range of 0.5µg-50µg protein
  • Flexible Protocols: Suitable for tube or Titer plate assays
  • Ready to use assay reagents and no preparation required
  • Long shelf life, stable for 12 months
Click for CB Protein Assay