Fractionation & Enrichment of Glycoproteins
For Multiple Downstream Applications, Including 2D Electrophoresis
For Multiple Downstream Applications, Including 2D ElectrophoresisRapid Separation of Glycoproteins Using Concanavalin A Spin Columns!
- Glycoproteins are proteins that are post-translationally modified by the addition of carbohydrates.
- Glycosylated proteins play critical roles in cell signaling, inflammation, cell-to-cell adhesion and in the immune response.
- FOCUS™ Glycoprotein rapidly fractionates glycoproteins that have terminal a-D-mannosyl and a-D glycosyl residues.
- FOCUS™ Glycoprotein utilizes spin columns containing the immobilized lectin Concanavalin A for rapid glycoprotein isolation.
Glycoproteins are proteins that are post-translationally modified by the addition of carbohydrates. The carbohydrates are coupled to asparagine (N-linked) and serine/ threonine (O-linked) residues during passage through the endoplasmic reticulum and golgi apparatus. They are commonly found decorating the cell membrane with the carbohydrate moieties in the extracellular space. Glycosylated proteins play critical roles in cell signaling, inflammation, cell-to-cell adhesion and in the immune response.
FOCUS™ Glycoprotein rapidly fractionates glycoproteins that have terminal a-D-mannosyl and a-D glycosyl residues. FOCUS™ Glycoprotein utilizes spin columns containing the immobilized lectin Concanavalin A for rapid glycoprotein isolation.
The aim of this application note is to evaluate FOCUS™ Glycoprotein kit in the fractionation of Jurkat cells. After fractionation, fractions were analyzed by 1D electrophoresis stained with Reversible Zinc Stain™ and 2D electrophoresis stained with a fluorescent protein stain.
Download the free application note to see FOCUS Glycoprotein in action.
